Potency Of Lignocellulose Degrading Bacteria Isolated From Buffalo And Horse Gastrointestinal Tract And Elephant Dung For Feed Fiber Degradation

摘要

Lignin is limiting factor for cellulose and hemicellulose degradation in rumen. Isolation andselection bacteria from buffalo and horse gastrointestinal tract and elephant dung could be foundbacteria that have superiority to degrade lignin, xylan, and cellulose. Those animals were chosenbecause they were herbivores that consume low quality crude fiber as their main energy sources.Lignocellulose degrading bacteria were isolated by Hungate selective media, by using lignin (tannicacid), xylan, and cellulose as selective substrates. The morphological identification used an enrichmentmedia by measuring color, colony size, diffusion zone, clear zone, and biochemical identification usingproduction of ligninase, xylanase, and cellulase enzymes. The best lignocellulose degrading bacteriathen was determined by the morphological and biochemical character. This study showed thatlignocellulose degrading bacteria could be found in gastrointestinal tract of buffalo and horse, andelephant dung. Highest number colony was found in samples from buffalo\u27s colon (376), followed byhorse\u27s cecum (203), elephant\u27s dung (46), buffalo\u27s cecum (23), buffalo\u27s rumen (9) and horse\u27s colon(7). The highest isolates activity of lignolytic, xylanolytic, and cellulolytic were reached by buffalo\u27scecum (7.64), horse\u27s cecum (6.27), and buffalo\u27s colon (2.48). Meanwhile the highest enzymesproductivities were: buffalo\u27s cecum (0.0400 µmol), horse\u27s cecum (1.3912 µmol) and buffalo\u27s colon(0.1971 µmol). Based on morphologycal character and biochemical test, it could be concluded thatlignolytic from buffalo\u27s cecum, xylanolytic from horse\u27s cecum, and cellulolytic from buffalo\u27s colonwere the superior isolates and they were 99% analyzed as Enterococcus casseliflavus/gallinarumspecies.